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Significance Actin plays essential roles in cellular processes such as muscle contraction, cell motility, and cytokinesis. Humans express six noninterchangeable actin isoforms. Mutations in all the isoforms have been linked… Click to show full abstract
Significance Actin plays essential roles in cellular processes such as muscle contraction, cell motility, and cytokinesis. Humans express six noninterchangeable actin isoforms. Mutations in all the isoforms have been linked to devastating human diseases. Despite its significance, there is no reliable method to produce fully native recombinant actin, featuring native posttranslational modifications and free of contamination from endogenous actin of expressing cells. We report a method to obtain high yields of recombinant actin in human cells that addresses the shortcomings of previous methods. This method opens the way to studies that address how differences among actin isoforms dictate their specialized cellular functions and will help define the molecular bases of disease-causing mutations, which should accelerate the development of targeted therapies.
Journal Title: Proceedings of the National Academy of Sciences of the United States of America
Year Published: 2022
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