LAUSR

Abstract There is currently no appropriate therapy for the treatment of injuries that affect the central nervous system. Therefore, scientists have attempted to explore novel methods to improve the neural tissue regeneration, while preventing inhibitory fibro glial scars. In these methods, hollow graphene oxide aerogel was initially made. Then, a porous gelatin structure was formed through the Thermal-Induced Phase Separation (TIPS) process, which was then filled the aerogel. Scanning Electron Microscope (SEM) was then employed to examine the effect of different parameters of GO solution concentration on the morphology of the aerogel. Next, mechanical-thermal characteristics of scaffolds were measured using Dynamic Mechanical Thermal Analysis (DMTA). At −70 °C, the mechanical strength of the gelatin scaffold sample and the hybrid scaffold is approximately 1.3 MPa and 22.6 MPa, respectively. There was a significant increase in the elastic modulus of the hybrid scaffold, indicating the effect of the aerogel reinforcement on the hybrid scaffold. For in vitro evaluation, P19 mouse cells were cultured and differentiated into nerve cells on hybrid scaffolds, followed by administrating immunofluorescence test. Cell differentiation was approximately 20 and 87% on the control surface and the scaffold surface, respectively, and the P19 cells were effectively differentiated into neural cells. Experimental results indicate that this model is suitable as a platform for neural tissue engineering. Graphical Abstract