LAUSR

Abstract A self-assembled functionalized metal-organic framework, Zn4O(NH2BDC)3 microcrystalline (IRMOF3) could be prepared by washing complex with DMF and CH2Cl2. Via modification with sodium alginate (AA), IRMOF3-AA samples could be obtained. IR and TEM were used for structural confirmation of IRMOF3-AA samples. PEG-GOD could be obtained via PEGylation of glucose oxidase (GOD), which could be confirmed by X-ray photoelectron spectroscopy (XPS) and used for further application. IRMOF3-AA@PEG-GOD samples could be obtained by the immobilization of PEG-GOD to IRMOF3-AA, and the best immobilization condition was achieved by adjusting the amount of GOD. From the view point of enzyme mimics, combined with peroxidase-like properties of the IRMOFS-AA samples, the determination of glucose was demonstrated with a linear range from 5 × 10−5 mol/L to 1.6 × 10−3 mol L−1 using tetramethylbenzidine (TMB) as chromogenic substrate. The prepared IRMOF3-AA@PEG-GOD samples exhibited dual functional enzyme-like (peroxidase and GOD-like) activities, with simple experimental steps.