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Separation of Monascus pigments from extractive fermentation broth with a high concentration of triton X-100

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ABSTRACT In this study, Monascus pigments were separated from the extractive fermentation broth by the combination of reverse micellar extraction and macroporous resins (MACR) adsorption with the alkalizing S-8 MACR… Click to show full abstract

ABSTRACT In this study, Monascus pigments were separated from the extractive fermentation broth by the combination of reverse micellar extraction and macroporous resins (MACR) adsorption with the alkalizing S-8 MACR (S-8 ALR). A 95.07% pigment adsorption was achieved together with a relative low Triton X-100 co-adsorption that was only 2.53%. The liquid intraparticle diffusion and film diffusion limit the pigment adsorption rate. A 79.48–84.03% of the pigment could be eluted from the resin using the methanol solution containing a 0.5% (w/v) hydrochloric acid. The S-8 ALR could be recovered for cycle use without a deficit of the adsorption or desorption efficiency. Abbreviations: ACR—Acidified resin; ALR—Alkalizing resin; HPLC—High performance liquid chromatography; PSM—pigment- surfactant micelle; TEM—Transmission electron microscopy.

Keywords: adsorption; monascus pigments; triton 100; extractive fermentation; fermentation broth

Journal Title: Separation Science and Technology
Year Published: 2018

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