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ABSTRACT The aim of study was to model and optimize the extraction of polyphenols and flavonoids with expressed antioxidant activity from chickpea seeds using a central composite design. The optimal… Click to show full abstract
ABSTRACT The aim of study was to model and optimize the extraction of polyphenols and flavonoids with expressed antioxidant activity from chickpea seeds using a central composite design. The optimal extraction conditions were the extraction time of 145.5 min, ethanol concentration of 83.7% and liquid-to-solid ratio of 24.9 (expressed as cm3 per g). The content of total polyphenol and flavonoid was 2.75 g gallic acid equivalent per kilogram of the dried extract and 0.147 g rutin equivalent per kilogram of the dried extract, respectively. Using 2,2-diphenyl-1-picrylhydrazyl assay, it was found that the optimal extract had the IC50 of 1.55 mg cm−3.
Journal Title: Separation Science and Technology
Year Published: 2019
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