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One step selective partition of ε-polylysine present in broth cultures in ionic liquid-based aqueous biphasic systems

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ABSTRACT Four different ionic liquids were synthesized, characterized and used with polypropylene glycol for the formation of aqueous biphasic systems and were used to selectively separate ε-PL from culture broth.… Click to show full abstract

ABSTRACT Four different ionic liquids were synthesized, characterized and used with polypropylene glycol for the formation of aqueous biphasic systems and were used to selectively separate ε-PL from culture broth. It was observed that the partition of ε-PL to the IL-rich phase was achieved, and with pure ε-PL, the extraction efficiency was found to be 99.92% while with culture broth, it was 93.42% for the ABS formed with 2-hydroxyethylammonium formate. Preservation of the chemical and structural stability of ε-PL in the ABSs indicated the potential of such systems as remarkable platform for the selective separation of ε-PL from the culture broth.

Keywords: aqueous biphasic; culture broth; biphasic systems; partition; one step

Journal Title: Separation Science and Technology
Year Published: 2020

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