LAUSR

Abstract Hyaluronic acid (HA) as a covering agent was incorporated into the ascorbic acid (AA)-niosomes to improve the performance of AA delivery systems into the skin. The preparation method: Thin film hydration. Characterisation tests: Field emission scanning electron microscopy, fourier transform infra-red spectroscopy, dynamic light scattering, UV-Visible, zeta potential, Franz diffusion cell, and flowcytometry. The niosomes with 10% w/w HA possessed the largest mean particle diameter of 341.0 ± 48.09 nm with PDI value of 0.29 ± 0.05, and the lowest zeta potential of −38.70 ± 0.27 mv. The drug encapsulation efficiency of this sample was 56.55 ± 0.99%, and in-vitro drug release test showed AA released in two slow and fast phases. Moreover, the highest amount of drug penetration and accumulation was related to this sample, recorded 116.55 ± 7.54 and 134.8 ± 10.04 µg/cm2, respectively. Niosomes coated with 10% w/w HA showed the greatest potential for improving the antioxidant activity of AA penetration into the skin.