LAUSR

PURPOSE To investigate the biochemical characteristics in experimental keratomycosis. METHODS Experimental mice were injected with Fusarium solanum solution Controls mice received liposomes containing phosphate-buffered saline (PBS-LIP). Raman spectroscopy was used to analyze the biochemical characteristics. The infiltration of inflammatory cells was analyzed by histopathology. Cytokine mRNA levels were detected by real-time polymerase chain reaction. RESULTS Raman Spectroscopy: In the experimental group, collagen, lipids, amide I and III were decreased, amide II, hyper proline amino acids, and arginine were increased, and proline and phenylalanine were significantly increased on day 3. Histopathology: more severe keratomycosis developed in the experimental group than in the control group.Statistically significant mRNA expression of Collagen4\MMP2\MMP9\TIMP1.MMP9 was negatively correlated with the secretion of Collagen4. CONCLUSIONS Matrix metalloproteinases are involved in biochemical changes in keratomycosis.