LAUSR

ABSTRACT The intact expression of core clock genes is essential for organism homeostatic regulation and Clock (Clk) as one of drivers in core circadian gene plays a crucial role to maintain circadian rhythm. In this study, we use GAL4/UAS system to downregulate the expression of Clk specifically in pigment-dispersing factor, a neuropeptide rhythmically released from small ventral lateral neurons (sLNvs) in the brain and combined with regular exercise. Then, we collected the activities using Drosophila activity monitor system and analyzed the sleep and activity status. The results showed that activity peaks in the morning and evening and total activities during the day and night of 6-week-old flies were significantly lower than those of 3-week-old flies in gene normal expression non-exercise group, and ineffective sleep increased during the day. The 3-week Clk RNAi Drosophila showed similar characteristics with 6-week normal flies in the weakened bimodal pattern activities; while they have worse sleep quality during the day, the 6-week Clk RNAi flies exhibited a reduced nighttime sleep volume and prolonged sleep latency. Although the regular exercise could not enhance the sleep quality of Clk RNAi Drosophila, it can significantly improve daytime sleep intensity by ameliorating age-related sleep inefficiency in normal group.