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Immunochromatographic strip for ultrasensitive detection of fumonisin B1

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ABSTRACT A sensitive monoclonal antibody (mAb) (1H1) against fumonisin B1 (FB1) was produced in our laboratory. A rapid, simple, and an immunochromatographic strip has been developed for the ultrasensitive detection… Click to show full abstract

ABSTRACT A sensitive monoclonal antibody (mAb) (1H1) against fumonisin B1 (FB1) was produced in our laboratory. A rapid, simple, and an immunochromatographic strip has been developed for the ultrasensitive detection of FB1 in corn samples. Under the optimized condition, the cut-off limits of test strips for FB1 were found to be 50 ng/mL in 0.01 M PBS and 25 ng/mL in corn samples. Results could be obtained within 5 min. The results revealed that the developed method is a rapid, sensitive, and simple tool for the detection of FB1.

Keywords: fumonisin; detection; strip ultrasensitive; ultrasensitive detection; immunochromatographic strip

Journal Title: Food and Agricultural Immunology
Year Published: 2018

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