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Optimization for microbial degradation of petroleum hydrocarbon (TPH) by Enterobacter sp. S-1 using response surface methodology

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Abstract In this research, the response surface method designed by Box-Behnken was used to study the effect of temperature, pH value and TPH concentration on the biodegradation of Enterobacter sp.… Click to show full abstract

Abstract In this research, the response surface method designed by Box-Behnken was used to study the effect of temperature, pH value and TPH concentration on the biodegradation of Enterobacter sp. S-1contaminated soil. The value of determination coefficient (R2=0.9565) by analysis of variance (ANOVA) indicated a satisfactory agreement between the quadratic model and the experimental data. It was found that TPH degradation rate was more significantly affected (P < 0.0006) by temperature compared with other two parameters. In the temperature of 30 °C, pH value of 7.14 and TPH concentration was 4.83 g/L, the best degradation rate was 81.63%.

Keywords: tph; degradation; methodology; response surface

Journal Title: Petroleum Science and Technology
Year Published: 2019

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