LAUSR

ABSTRACT The nutritional value of the rhizomes of Kaempferia angustifolia was measured through the antioxidant properties of various extracts and the determination of the bioactive compounds. The chloroform and methanol extracts of the rhizomes of Kaempferia angustifolia showed strong free radical scavenging activities against 1,1-diphenyl-2-picrylhydrazyl (DPPH) with values of 615.92 mg Trolox equivalent (TE)/g each. The methanol extract also exhibited the strongest antioxidant properties in the azinobis (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) assay with a value of 38.87 mg TE/g. The hexane extract gave the strongest reducing ability in cupric reducing antioxidant capacity (CUPRAC) assay with a value of 901.76 mg TE/g, whilst the ethyl acetate extract exhibited the strongest reducing ability against ferric reducing antioxidant power (FRAP) with a value of 342.23 mg TE/g. Column chromatographic separation on the rhizomes of Kaempferia angustifolia afforded boesenboxide (1), crotepoxide (2), 2ˊ-hydroxy-4,4ˊ,6ˊ-trimethoxychalcone (3), kaempfolienol (4), and zeylenol (5), which were elucidated by spectroscopic methods. Kaempfolienol (4) was the strongest free radical agent against DPPH with a value of 443.92 mg TE/g, whilst 2ˊ-hydroxy-4,4ˊ,6ˊ-trimethoxychalcone (3) exhibited the strongest antioxidant properties with values of 42.23, 1497.22, and 781.53 mg TE/g against ABTS, CUPRAC, and FRAP assays, respectively.