LAUSR

Abstract The excessive concentration of multiple heavy metals in the tannery solid waste (TSW) needs integrated process solutions for its decontamination. This study is aimed at deriving TSW compost and autochthonous microbe synergies for improving phytoextraction potential of sunflower. In-vessel composting of TSW was carried out by using fruit waste as an inoculum to achieve the optimized conditions. Autochthonous strains of Trichoderma viride and Bacilllus sp. isolated from TSW were utilized individually as well as in combination with TSWC amendments of 2.5, 5 and 10% (w/w) prepared in our pilot scale experiment. Analyses of TSW compost based on FTIR and SEM illustrated the wide range of functionality and porosity along the mesh of fungal hyphae and inorganic moieties present on the compost surface. Plant biomass and TMs uptake (Cr 540 mg kg−1 > Cd 330 mg kg−1 > Pb 285 mg kg−1) were significantly pronounced in shoots of sunflower under combined treatments at 10% TSWC amended soils. However, in seeds, TMs were found below detection limit (BDL) through atomic absorption spectrophotometry. Biochemical assays of sunflower including total chlorophyll content (18%), total soluble protein (45%), superoxide dismutase (80%) and catalase (75%) activities were also increased significantly at higher level of amendment in combination with microbes than in the control. Despite being high in TMs, high biomass in sunflower and associated elevation in biochemical products demonstrate the potential of TSW for valorization. Novelty statement: This study identifies the cost-effective management of multi metal contaminated tannery solid waste through deriving its compost along with autochthonous microbes as phytoextraction assistants by yielding higher plant biomass. This study suggests the use of composted TSW inoculated with selected autochthonous fungi and bacteria for enhancing sunflower’s biomass and enhancing the bioavailable fractions of toxic metals for phytoextraction.