LAUSR

Abstract Pleurotus ostreatus (P. ostreatus) and Lentinus subnudus (L. subnudus) have been used by the locals for the management of Alzheimer’s disease (AD) but with scant scientific sources. The aim of this study is to assess the neuroprotective properties of P. ostreatus and L. subnudus using transgenic Drosophila melanogaster flies (TDMF). The activity of acetylcholinesterase (AChE), butyrylcholinesterase (BChE), as well as the antioxidant status of TDMF raised on a diet supplemented with P. ostreatus and L. subnudus were determined. The flies were raised on a diet devoid of supplements or supplemented with P. ostreatus or L. subnudus (1% and 5% inclusion) for 7 days. Afterward, AChE and BChE activities, as well as catalase and total thiol level, were determined. The reactive oxygen species (ROS) and malondialdehyde (MDA) levels were also determined in the flies raised on a diet devoid of supplement and on supplemented diets. Meanwhile, flies raised on P. ostreatus– and L. subnudus–supplemented diets exhibited a significant reduction in the activity of AChE and BChE in comparison with the controls. Also, supplemented diets significantly (p < 0.05) enhance catalase activity and improve total thiol level, while ROS and MDA levels were observed to be reduced in all the flies raised on the supplemented diets in comparison with the controls. In summary, reduction in the activity of AChE and BChE, as well as improved antioxidant status in TDMF, could be some of the mechanisms through which P. ostreatus and L. subnudus exhibit anti-AD properties. Nevertheless, L. subnudus exhibits a better neuroprotective effect than P. ostreatus.