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Molecular detection of bacteria associated with Serendipita sp., a mycorrhizal fungus from the orchid Stanhopea connata Klotzsch in southern Ecuador

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Abstract We examined the bacterial community associated with an orchid mycorrhizal fungus from the Neotropical region. Living hyphae of Serendipita sp., isolated from roots of Stanhopea connata, were screened using… Click to show full abstract

Abstract We examined the bacterial community associated with an orchid mycorrhizal fungus from the Neotropical region. Living hyphae of Serendipita sp., isolated from roots of Stanhopea connata, were screened using molecular tools to identify associated prokaryotes. In total, nine distinctive bacterial OTUs were detected belonging to phyla Firmicutes, Proteobacteria and Acidobacteria. Within Firmicutes the genera Bacillus, Gemella, Staphylococcus and Streptococcus were detected. Other genera such as Paracoccus, Achromobacter from Proteobacteria and Acidobacter from Acidobacteria were also revealed. Additionally, one OTU with close similarity to Neisseriaceae of the Betaproteobacteria was detected. Further investigations, eg fluorescent or electron microscopy, are necessary to determine whether the uncovered bacteria are situated inside or outside the fungal hyphae.

Keywords: detection bacteria; molecular detection; stanhopea connata; bacteria associated; mycorrhizal fungus

Journal Title: Botany Letters
Year Published: 2018

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