LAUSR

Angle-resolved light scattering measurements of human metaphase chromosomes were compared to the results of numerical light scattering simulations with geometrical models based on atomic force microscopy (AFM) measurements of the same chromosomes. The simulations were conducted using the discrete dipole approximation method (DDA), which solves Maxwell's equations for induced dipoles, positioned in a discrete lattice. A remarkable agreement between the light scattering simulations and measurements of all 6 studied chromosomes was found. Additionally, the influence of small changes in the orientation of a complex scatterer geometry on its angle-resolved scattering pattern is shown. A method is presented to approximate such variations in the scatterer's orientation by a linear shift of the angular scattering pattern. This method provides an initial guess on the scatterers orientation, reducing the amount of simulations needed considerably. It was validated on simulations of a cuboid and successfully applied in the evaluation of the chromosome measurements.