LAUSR

The present work demonstrated a novel biological method of synthesising gold nanoparticles using aqueous extract of Eupatorium odoratum leaf extract. The method has a good control over the size and shape of the particles. The biochemicals present in the leaf extract act as effective reducing agent and capping agent. Synthesised gold nanoparticles were characterised by UV–vis spectrometer, transmission electron microscope and zeta potential analyser. TEM study revealed that particles are highly monodispersed with isotropic and anisotropic shapes corresponding to two different sets of reaction procedures. Two different sets of gold nanoparticles were used to check their cellular uptake and localisation without any functionalisation. Inductively coupled plasma mass spectrometry (ICP-MS) and TEM were used to quantify and visualise intracellular gold nanoparticle content after exposure to in vitro mice spleen cell lines. The study reveals the uptake of both types of gold nanoparticles by the spleen cells. The triangular shaped nanoparticles were observed in large extent in comparison to quasi-spherical gold nanoparticles.