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Isolation Methods of Peripheral Blood Mononuclear Cells in Spanish Biobanks: An Overview.

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The Spanish Hematic Derivatives Group, consisting of 26 biobanks, was established in 2011. We describe here the viability results of our publically available standard operating procedure to freeze and thaw… Click to show full abstract

The Spanish Hematic Derivatives Group, consisting of 26 biobanks, was established in 2011. We describe here the viability results of our publically available standard operating procedure to freeze and thaw peripheral blood mononuclear cells (PBMCs). Our protocol maximizes PBMC viability while avoiding where possible interbiobank and intrabiobank assay variability.

Keywords: blood mononuclear; peripheral blood; isolation methods; mononuclear cells

Journal Title: Biopreservation and biobanking
Year Published: 2017

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