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AAV vectors are a powerful tool for gene transfer approaches. We have established a simple and fast plasmid-based production system for achieving high AAV titers within 6 working days. The… Click to show full abstract
AAV vectors are a powerful tool for gene transfer approaches. We have established a simple and fast plasmid-based production system for achieving high AAV titers within 6 working days. The same procedure can be used for all serotypes and thus allows direct comparability of different serotypes. In this protocol we describe a step-by-step procedure which results in well-characterized vectors suitable for both in vitro approaches and preclinical studies.
Keywords:
aav;
protocol efficient;
efficient generation;
characterization adeno;
aav vectors;
generation characterization
Journal Title: Human gene therapy methods
Year Published: 2017
Link to full text (if available)
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Journal Title: Human gene therapy methods
Year Published: 2017
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!