LAUSR

Abstract The objective of this study was to evaluate the removal capacity of bacterial film formed by Pseudomonas sp., using commercial enzymes (pectinase, cellulase, and amylase) and the enzymatic extract obtained by solid-state cultivation (SSC) from Aspergillus niger ATCC 9642. The bacterial biofilm removal (Pseudomonas sp.) was evaluated using the commercial enzyme microplate technique and the crude enzyme extract (SSC). The effects of orange peel (9.28–22.72 g), corn steep liquor (0.32–3.68 g), and wheat bran (0–4 g) concentrations were evaluated on pectinase production in solid-state cultivation. The enzyme extract (SSC) satisfactorily removed 77 % of biofilm after 10 min contact, taking into account that there was no enzymatic extract purification treatment. The results indicated a promising and economically viable alternative to enzymatic removal of biofilms in the food industry.