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Live-cell single-molecule labeling and analysis of myosin motors with quantum dots

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Quantum dots (QDs) are a powerful tool for quantitative biology, but two challenges are associated with using them to track intracellular molecules in live cells. A simple and convenient method… Click to show full abstract

Quantum dots (QDs) are a powerful tool for quantitative biology, but two challenges are associated with using them to track intracellular molecules in live cells. A simple and convenient method is presented for labeling intracellular molecules by using HaloTag technology and electroporation and is used to successfully track myosins within live cells.

Keywords: single molecule; live cell; cell single; biology; quantum dots

Journal Title: Molecular Biology of the Cell
Year Published: 2017

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