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This work describes an elegant approach for direct, site-specific labeling of proteins with fluorescent-dyes for live cell imaging. By integrating a noncanonical amino acid that is capable of binding a… Click to show full abstract
This work describes an elegant approach for direct, site-specific labeling of proteins with fluorescent-dyes for live cell imaging. By integrating a noncanonical amino acid that is capable of binding a fluorescent dye into tubulin, we directly and specifically labeled tubulin with a fluorescent-dye and imaged microtubules in live mammlian cells.
Keywords:
direct fluorescent;
cell;
fluorescent dye;
live cell
Journal Title: Molecular Biology of the Cell
Year Published: 2017
Link to full text (if available)
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Journal Title: Molecular Biology of the Cell
Year Published: 2017
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!