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Conventional nonhomologous end joining–based gene knockouts can lead to the production of C-terminally truncated proteins with potentially residual or dominant negative functions. Combining CRISPR/Cas9 with gene traps targeting the first… Click to show full abstract
Conventional nonhomologous end joining–based gene knockouts can lead to the production of C-terminally truncated proteins with potentially residual or dominant negative functions. Combining CRISPR/Cas9 with gene traps targeting the first intron (CRISPR-Trap), however, completely prevents the expression of the open reading frame, resulting in clean gene knockouts.
Keywords:
gene knockouts;
approach generation;
clean approach;
trap clean;
gene;
crispr trap
Journal Title: Molecular Biology of the Cell
Year Published: 2018
Link to full text (if available)
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Journal Title: Molecular Biology of the Cell
Year Published: 2018
Link to full text (if available)
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recommendations!