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MOTIVATION Single-molecule localization microscopy resolves individual fluorophores or fluorescence-labeled biomolecules. Data is provided as a set of localizations that distribute normally around the true fluorophore position with a variance determined… Click to show full abstract
MOTIVATION Single-molecule localization microscopy resolves individual fluorophores or fluorescence-labeled biomolecules. Data is provided as a set of localizations that distribute normally around the true fluorophore position with a variance determined by the localization precision. Characterizing the spatial fluorophore distribution to differentiate between resolution-limited localization clusters, which resemble individual biomolecules, and extended structures, which represent aggregated molecular complexes, is a common challenge. RESULTS We demonstrate use of the convex hull and related hull properties of localization clusters for diagnostic purposes, as a parameter for cluster selection, or as a tool to determine localization precision. AVAILABILITY https://github.com/super-resolution/Ebert-et-al-2022-supplement. SUPPLEMENTARY INFORMATION Supplementary data are available at Bioinformatics online.
Journal Title: Bioinformatics
Year Published: 2022
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