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Chainy, an universal tool for standardized relative quantification in real-time PCR.

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Chainy is a cross-platform web tool providing systematic pipelines and steady criteria to process real-time PCR data, including the calculation of efficiencies from raw data by kinetic methods, evaluation of… Click to show full abstract

Chainy is a cross-platform web tool providing systematic pipelines and steady criteria to process real-time PCR data, including the calculation of efficiencies from raw data by kinetic methods, evaluation of the suitability of multiple references, standardized normalization using one or more references, and group-wise relative quantification statistical testing. We illustrate the utility of Chainy for differential expression and chromatin immunoprecipitation enrichment (ChIP-QPCR) analysis. AVAILABILITY Chainy is open source and freely available at http://maplab.cat/chainy. CONTACT [email protected] information: Supplementary data are available at Bioinformatics online.

Keywords: time pcr; relative quantification; real time; tool

Journal Title: Bioinformatics
Year Published: 2017

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