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Multispectral tracing in densely labeled mouse brain with nTracer

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Abstract Summary This note describes nTracer, an ImageJ plug-in for user-guided, semi-automated tracing of multispectral fluorescent tissue samples. This approach allows for rapid and accurate reconstruction of whole cell morphology… Click to show full abstract

Abstract Summary This note describes nTracer, an ImageJ plug-in for user-guided, semi-automated tracing of multispectral fluorescent tissue samples. This approach allows for rapid and accurate reconstruction of whole cell morphology of large neuronal populations in densely labeled brains. Availability and implementation nTracer was written as a plug-in for the open source image processing software ImageJ. The software, instructional documentation, tutorial videos, sample image and sample tracing results are available at https://www.cai-lab.org/ntracer-tutorial. Supplementary information Supplementary data are available at Bioinformatics online.

Keywords: mouse brain; labeled mouse; tracing densely; brain ntracer; densely labeled; multispectral tracing

Journal Title: Bioinformatics
Year Published: 2019

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