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In this study, we studied the chromatographic performance of this newly developed wide pore superficially porous particles (SPPs) with 3.5 μm particle size and 450 Å pore size, for the… Click to show full abstract
In this study, we studied the chromatographic performance of this newly developed wide pore superficially porous particles (SPPs) with 3.5 μm particle size and 450 Å pore size, for the separation of proteins and Immunoglobulin G antibodies. We studied the selectivity of different phases (C4, SB-C18 and Diphenyl), the effect of temperature, column carryover and column chemical lifetime. We also compared our SPPs with other wide pore SPPs in similar particle sizes and sub 2 µ wide pore totally porous particles by van Deemter studies and gradient separations of proteins and immunoglobulin G antibodies. The results showed that the SPPs containing larger pore size gave better chromatographic performance.
Journal Title: Journal of chromatographic science
Year Published: 2018
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