LAUSR

The parasitoid wasp, Trichogramma pintoi, is a promising candidate for inundative release against Heortia vitessoides. Parasitoid females can regulate the sex of their offspring in response to environmental and biological factors. In pest control programs utilizing these parasitoids, male overproduction is not conducive to success. To optimize the production of T. pintoi as an egg parasitoid of H. vitessoides, factors affecting the rates of parasitism and eclosion and the percentage of females among T. pintoi offspring, such as temperature, photoperiod, host age, host density, maternal age, maternal density, and food, were investigated. The proportion of T. pintoi female offspring was significantly affected by temperature, photoperiod, host density, maternal age, and maternal density. The female offspring percentage decreased in response to host density (160 eggs), maternal age (≥ 4 days old), maternal density (≥ 4 females), photoperiods (24:0 and 18:6 L:D), and extremely low temperature (15 °C). However, host age and female diet did not affect the proportion of female offspring. According to the present work, female parasitoid production can be maximized under laboratory conditions of 25 °C, 75% relative humidity, and a photoperiod of 0:24 h (L:D) via exposure of forty 1-day-old H. vitessoides eggs for 24 h or eighty 1-day-old H. vitessoides eggs to a newly emerged, mated female fed a 10% sucrose solution until the female dies. These findings will guide mass production efforts for this parasitoid.