LAUSR

A simple and facile way of using biogenic silver nanoparticles (BSNP) (10-20 nm) were fabricated for wound healing acceleration and suppressing wound infections. The BSNP were formulated in the ointment base and conducted the study to accelerate the wound healing process on rat. The BSNP ointment pH 6.8 ± 0.5 lies in normal pH range of the human skin with a good spreadability and diffusibility. The percentage of wound closure rate was highest at day 3 of BSNP ointment treated wound by 22.77 ± 1.60%, while in untreated control 10.99 ± 1.74%, Betadine 14.73 ± 2.36% and Soframycin 18.55 ± 1.37% compared to day 0. The similar pattern was found at day 7 and 11. The antibacterial activity of BSNP was evaluated against wound infection causing bacteria Staphylococcus aureus, Pseudomonas aeruginosa and E. coli by agar diffusion method. The total bacterial counts on wound area were enumerated by colony forming unit method. The lowest number of bacterial counts was found in BSNP treated wound than other groups. BSNP treatment at 7.5% concentration enhanced migration of fibroblasts in scratch assay. These findings reveals the BSNP as an efficient contrivance for wound healing acceleration and antimicrobial agent for alternative therapeutic need in eco-friendly manner.