LAUSR

Although only 6 years old, the CRISPR system has blossomed into a tool for rapid, on-demand genome engineering and gene regulation in Saccharomyces cerevisiae. In this mini-review, we discuss fundamental CRISPR technologies, tools to improve the efficiency and capabilities of gene targeting, and cutting edge techniques to explore gene editing and transcriptional regulation at genome-scale using pooled approaches. The focus is applications to metabolic engineering with topics including development of techniques to edit the genome in multiplex, tools to enable large numbers of genetic modifications using pooled sgRNA libraries, and efforts to enable programmable transcriptional regulation using endonuclease-null Cas enzymes.