LAUSR

The dawn of the computer and information age in the last century left virtually no field untouched. In biology, computational advances enabled scientists to generate, store, and analyze large-scale data sets that could scarcely have been imagined decades earlier. These advances ultimately led to the publication of the first bacterial genome sequence in 1995 (Fleischmann et al. 1995), and with it, the birth of the genomics era. The advent of high-throughput sequencing further accelerated the pace of data generation to an unprecedented rate. Now, less than a quarter of a century later, genomic data for almost 220,000 individual organisms and another 25,000 metagenomes are currently available through the National Center for Biotechnology Information (NCBI) website, and Genome Biology and Evolution has played a role in publishing numerous articles in the field of computational evolutionary biology. With this wealth of widely available sequence data, the challenge for evolutionary biologists has become how to analyze genomic data sets to answer new questions and reveal new evolutionary insights. Today’s computational biologists do this by applying theoretical methods, mathematical modeling, and computational simulations in novel ways. They advance the field of evolutionary biology by providing tools and methods that can be used to gain insight into evolutionary processes in a variety of systems and at multiple scales. Genome Biology and Evolution’s virtual issue on computational biology highlights some of these new approaches that have been published in the journal over the last 3 years. Some of these computational methods have practical uses in neighboring fields of biology. For example, in their article, “In Silico Identification of Candidate Genes for Fertility Restoration in Cytoplasmic Male Sterile Perennial Ryegrass (Lolium perenne L.),” Sykes et al. (2017) present a pipeline for identifying candidate restorer of fertility (Rf) genes in any plant species. This is a key element in strategies that attempt to use hybrid breeding to increase crop yield, and this method, according to the authors, “provide[s] plant breeders with a molecular tool for candidate Rf gene identification and thus facilitate[s] the implementation of hybrid breeding schemes.” Other articles in the issue present computational tools designed to improve and advance genomic analysis. In “IMPUTOR: Phylogenetically Aware Software for Imputation of Errors in Next-Generation Sequencing,” Jobin et al. (2018) present software that improves the completeness and accuracy of next-generation sequence data. Their method uses phylogenetic information and the principle of parsimony to correct errors and impute missing bases due to low coverage. This is especially important given recent evidence of errors in sequence databases due to DNA damage/mutagenic processes (Chen et al. 2017). Duchemin et al. (2017) present a tool for genomic analysis on a more macro level in their paper, “DeCoSTAR: Reconstructing the Ancestral Organization of Genes or Genomes Using Reconciled Phylogenies.” Building on earlier work, this software reconstructs putative ancestral states of genomic “adjacencies,” that is, genomic features that are adjacent in the genome. Importantly, these adjacencies can be studied at virtually any level, so that DeCoSTAR can be used for investigating “ancestral domain structures of a modular protein, as well as chromosome organizations of whole ancestral genomes, or fusion/fission histories or modular genes.” Additional tools that promise to expand the possibilities of genomic analysis include MultiTwin (“MultiTwin: A Software Suite to Analyze Evolution at Multiple Levels of Organization Using Multipartite Graphs”) from Corel et al. (2018), which allows for the “integration of several levels of biological organization (genes, genomes, communities, environments) [for] more comprehensive analyses of gene sharing and improved sequence-based classifications.” Furthermore, Wang et al. (2017) describe a method for identifying allele-specific gene expression in natural populations in their paper, “Bayesian Inference of Allele-Specific Gene Expression Indicates Abundant Cis-Regulatory Variation in Natural Flycatcher Populations.” In addition to the above tools, which promise to improve the accuracy, power, and potential of genomic analyses, the virtual issue highlights studies that provide new insight into