LAUSR

Sir, Burkholderia pseudomallei is a biothreat agent and causes melioidosis, a globally emerging infectious disease with high case fatality rates, and B. pseudomallei infections are difficult to treat. Although curiously rare in B. pseudomallei, drug resistance is observed and primarily due to efflux, enzymatic modification or target mutation. Efflux is the dominant resistance mechanism. Melioidosis treatment requires an acute-phase therapy limited mostly to b-lactam antibiotics such as ceftazidime and carbapenems, followed by a prolonged eradication-phase therapy, which nowadays is mostly limited to trimethoprim/sulfamethoxazole. While extensively employed for treatment of various Gramnegative infections, several studies have shown that fluoroquinolones are not useful for melioidosis therapy. A previous study published in this Journal showed that the in vitro activity of ciprofloxacin against B. pseudomallei is very weak and the activity of newer fluoroquinolones such as gatifloxacin and moxifloxacin is rather limited. The reasons for therapeutic inefficacy of fluoroquinolones with B. pseudomallei are unknown. Acquired fluoroquinolone resistance in B. pseudomallei predominantly involves expression of the BpeEF-OprC efflux pump, although target mutations affecting the quinolone resistance-determining region of GyrA have been reported. Finafloxacin is a fluoroquinolone that was approved by the US FDA in 2014 for the treatment of Pseudomonas aeruginosa-mediated acute otitis externa. A unique property of finafloxacin is that it exhibits increased activity at acidic pH and, when compared with other fluoroquinolones, superior antibacterial activity in acidic conditions (e.g. pH 5.8). These attributes support the notion that the drug is suitable for the treatment of infections in acidic environments. B. pseudomallei is an intracellular pathogen that can replicate within host organelles where the local pH is acidic (e.g. in macrophages). A previous study indicated that finafloxacin possesses potent bactericidal activity against B. pseudomallei, and bacterial burdens 24 h postchallenge were lower in finafloxacin-treated animals than in those treated with ciprofloxacin or trimethoprim/sulfamethoxazole. Because efflux is an inherent liability of fluoroquinolones in Gram-negative bacteria, in this study we compared the in vitro efficacy of finafloxacin using a defined, isogenic panel of efflux-proficient or efflux-compromised B. pseudomallei mutants based on prototype strain 1026b. B. pseudomallei encodes 10 efflux pumps of the resistance nodulation cell-division (RND) family, but only three of these (AmrAB-OprA, BpeAB-OprB and BpeEF-OprC) have been characterized in some detail. AmrAB-OprA is expressed at significant levels in wild-type strains (e.g. 1026b; Table 1), where it is responsible for the intrinsic aminoglycoside and macrolide resistance observed in the vast majority of clinical and environmental isolates. BpeAB-OprB is expressed at very low levels in wild-type strains and de-repressed in bpeR regulatory mutants (e.g. Bp58). BpeAF-OprC is not expressed in wild-type strains, but up-regulated in bpeT regulatory mutants (e.g. Bp282). Whereas AmrAB-OprA and BpeEF-OprC are expressed in MDR clinical isolates, the clinical significance of BpeAB-OprB remains yet to be established. Although the BpeEF-OprC pump is known to be the major fluoroquinolone resistance mechanism in B. pseudomallei, we included BpeAB-OprB mutants in our studies because when expressed this pump confers low-level fluoroquinolone resistance. MIC values of ciprofloxacin and the comparators moxifloxacin and finafloxacin were determined using Merlin Micronaut-S microtitre plates and broth microdilution. B. pseudomallei efflux-proficient and effluxdeficient strains, including prototype 1026b, were grown to midlog phase in cation-adjusted Mueller–Hinton II broth (MHB; Becton Dickinson and Company, Sparks, MD, USA) and inoculated into MHB adjusted to pH 5.8 or 7.2. Growth was visually inspected after 20 h of incubation at 37 C. Finafloxacin MIC values were generally lower than those of ciprofloxacin and moxifloxacin at pH 7.2, and substantially lower at pH 5.8. All three antibiotics appear to be extruded to some extent by the BpeAB-OprB and BpeEF-OprC efflux pumps, but not AmrAB-OprA. The data confirm that BpeEF-OprC is a major fluoroquinolone resistance mechanism in B. pseudomallei, which bestows high-level ciprofloxacin and moxifloxacin resistance. At pH 7.2 the MICs of both fluoroquinolones are 2 mg/L for 1026b versus 32 mg/L (ciprofloxacin) and 16 mg/L (moxifloxacin) for the BpeEF-OprC-expressing strain Bp282. While the finafloxacin MIC value is higher for the BpeEF-OprC-expressing strain when compared with the 1026b prototype control (2 versus 0.25 mg/L at pH 7.2 and 2 versus 0.125 mg/L at pH 5.8, respectively), the susceptibility of B. pseudomallei to finafloxacin is less affected by efflux than ciprofloxacin and moxifloxacin, especially at acidic pH. In summary, our data show that finafloxacin maintains in vitro efficacy against B. pseudomallei in the face of efflux-