LAUSR

AIMS The purpose of this study was to evaluate the effects of altered environmental conditions, specifically elevated temperature at various levels of expected relative humidity (RH), on the inactivation of SARS-CoV-2 when applied to U.S. Air Force aircraft materials. METHODS AND RESULTS SARS CoV-2 (USA-WA1/2020) was spiked (approximately 1×105 TCID50) in either synthetic saliva or lung fluid, dried onto porous (e.g., Nylon strap) and nonporous materials (e.g., bare aluminum, silicone, and ABS plastic), placed in a test chamber and exposed to environmental conditions ranging from 40-51.7°C and RH ranging from 0-50%. The amount of infectious SARS-CoV-2 was then assessed at various timepoints from 0-2 days. Warmer test temperatures, higher RH, and longer exposure duration resulted in higher inactivation rates per material type. Synthetic saliva inoculation vehicle was more readily decontaminated compared to materials inoculated with synthetic lung fluid. CONCLUSIONS SARS-CoV-2 was readily inactivated below limit of quantitation (LOQ) for all materials inoculated using synthetic saliva vehicle within 6 hours when exposed to environmental conditions of 51.7°C and RH ≥ 25%. Synthetic lung fluid vehicle did not follow the general trend of an increase in RH resulting in increased efficacy. The lung fluid performed best at the 20-25% RH range to achieve complete inactivation below LOQ.