LAUSR.org creates dashboard-style pages of related content for over 1.5 million academic articles. Sign Up to like articles & get recommendations!

A Lateral Flow Method for Aflatoxin B1 in Dry Dog Food: An Inter-Laboratory Trial.

Photo from wikipedia

BACKGROUND Dogs are highly susceptible to aflatoxins, the mycotoxins which most commonly cause acute dog illnesses and deaths following consumption of contaminated food. OBJECTIVE In this study, a screening method… Click to show full abstract

BACKGROUND Dogs are highly susceptible to aflatoxins, the mycotoxins which most commonly cause acute dog illnesses and deaths following consumption of contaminated food. OBJECTIVE In this study, a screening method to detect aflatoxin B1 (AFB1) in dry dog food was further evaluated at the FDA action level of 20 ng/g. A fourth round multi-laboratory trial was performed. In contrast to the previous work, a different source of dog food was used in the multi-laboratory trial and more participants were involved. METHOD The tested lateral flow method employs a modified procedure of the "Rosa® AFQ-Fast Test Kit" from Charm Sciences Inc. A total of 60 unfortified blank study samples, 220 study samples fortified at 20 ng/g, and 80 study samples fortified at 9-11 ng/g were prepared by an independent party and analyzed in ten collaborating laboratories in a blinded manner. RESULTS The pass rates were 98.3% and 94.5% for unfortified and 20 ng/g fortified study samples, respectively. CONCLUSIONS The method is suitable for aflatoxin B1 screening at the FDA action level of 20 ng/g in a complex matrix such as dry dog food. HIGHLIGHTS This work completes extensive method performance evaluation through four rounds of multi-laboratory trials.

Keywords: dry dog; dog food; laboratory trial; study; food; dog

Journal Title: Journal of AOAC International
Year Published: 2021

Link to full text (if available)


Share on Social Media:                               Sign Up to like & get
recommendations!

Related content

More Information              News              Social Media              Video              Recommended



                Click one of the above tabs to view related content.