LAUSR

BACKGROUND Sulphasalazine as a prodrug, its active metabolite (mesalazine) which also is available in pharmaceutical formulations, and the major active metabolite of mesalazine; N-acetyl-5-aminosalicylic aid are commonly used for treatment of inflammatory bowel diseases. OBJECTIVES Two accurate, precise, sensitive and specific spectrophotometric methods were developed and validated for determination of the studied components. METHODS The first method was modified ratio difference spectrophotometric method. In this method, SZ was determined by measuring the peak area from 410-500 nm, while MZ and AS were determined by measuring the difference of the selected amplitudes values. The second one was mean centering of ratio spectra spectrophotometric method. RESULTS The developed method were linear over the concentration range of (2-35), (2-30) and (1-25) µg/mL for sulphasalazine, mesalazine, and N-acetyl-5-aminosalicylic acid, respectively. CONCLUSION The developed methods were validated according to ICH guidelines. They were successfully applied for determination of studied analytes. Greenness assessment was evaluated using three different tools. HIGHLIGHTS Spectrophotometric methods were developed for determination of SZ and its related compounds for the first time. They were designated to be green and ecofriendly ones and their greenness profiles were evaluated using green solvents to keep the environment clean.