LAUSR

BACKGROUND A micellar-HPLC method was developed for the determination of oxaliplatin (OHP) and curcumin (CUR) employing a C-18 column (4.6 × 250 mm, dp = 5 µm) and diode array detector. Objective: A rapid, cost-effective, environmentally friendly, less time-consuming, easy-to-handle, safe method was developed. Methods: The conditions were optimized for the estimation of OHP and CUR, i.e., 0.15 mol/L sodium dodecyl sulfate (SDS) -6% (v/v) pentanol buffered to pH 5.0 with a flow rate of 1.0 mL/min, injection volume of 20 µL, and detection at 325 nm. Different analytical parameters, like linearity, accuracy, precision, robustness, specificity, LOD, and LOQ, were determined in compliance with the ICH guidelines. Results: The LOD (S/N = 3) of OHP was 0.004 µg/mL; on the contrary, it was 0.005 µg/mL for CUR. The calibration curves for OHP and CUR depicted linearity over the ranges 0.015-10 µg/mL (determination coefficient i.e., r2 = 0.9999) and 0.015-10 µg/mL (r2 = 0.9994), respectively. Conclusion: The drugs were eluted in < 12 min and the developed method was applicable for analyzing multiple samples per day. Moreover, it was determined to be robust and was used to quantify OHP and CUR in mice serum/blood. It could pave the way for quantitative analysis of these drugs during the development of a pharmaceutical preparation for the treatment of colorectal cancer. HIGHLIGHTS A simple, cost-effective, eco-friendly HPLC method was developed to simultaneously estimate oxaliplatin and curcumin. The developed method was validated as per the ICH guidelines.