LAUSR

BACKGROUND Hypertension is a key risk factor for ischemic heart disease and atherosclerosis. Most patients require a combination of antihypertensive medications to accomplish their therapeutic goals. Antihypertensive medicines such as calcium channel blockers and angiotensin receptor blockers are indicated for patients whose high blood pressure cannot be controlled with monotherapy. The combination of Amlodipine besylate (AML) with Irbesartan (IRB) is an example of this synergistic activity in lowering blood pressure. OBJECTIVE In this regard, the goal of the research is to develop sensitive spectrophotometric methods for the simultaneous determination of Amlodipine besylate and Irbesartan. METHODS Three simple ratio spectra manipulating spectrophotometric methods namely, ratio difference, mean centering of ratio spectra and derivative ratio were developed for the simultaneous assay of the cited mixture. RESULTS Linear correlations were attained over the concentration range of 1-35 μg/mL and 2-35 μg/mL for Amlodipine besylate and Irbesartan, respectively. The methods were validated according to the ICH guidelines with good results. CONCLUSION Eventually, the introduced methods were successfully applied for the assay of the cited drugs in their marketed formulation. They could be efficiently used for routine analysis of the mentioned drugs in quality control laboratories. HIGHLIGHTS The proposed approaches do not require expensive solvents or complex instruments. They could be used in routine laboratory tests where the time and cost are crucial.