LAUSR

BACKGROUND There are reports for the simultaneous estimation of sildenafil (SIL) and tramadol (TM) using chromatographic techniques. Some limitations of these methods are high cost and time, more usage of the pollutant solvents, and complicated instruments. OBJECTIVE Simultaneous determination of SIL and TM in the anti-sexual dysfunction pharmaceutical formulation using rapid and smart chemometrics approaches along with spectrophotometry. METHODS In this study, spectrophotometric analysis method based on continuous wavelet transform (CWT), partial least squares (PLS) and radial basis function neural network (RBF-NN) were presented. RESULTS In the CWT method, the Symlet (Sym2) and Daubechies (db2) wavelet families with wavelengths of 268 nm and 221 nm were considered as the best families for SIL and TM, respectively. The limit of detection (LOD) and limit of quantitation (LOQ) values were found to be 0.0185, 0.0054 μg/mL and 0.0458, 0.3092 μg/mL for SIL and TM, respectively. The mean recovery values were 101.06% and 101.49% for SIL and TM, respectively. The results of PLS revealed that the root mean square error (RMSE) of SIL and TM were 0.0286 and 0.0635, respectively. Also, the mean recovery of both drugs was obtained over 99%. The mean square error (MSE) of RBF-NN was 5.88 × 10-29 and 4.61 × 10-27 for the SIL and TM, respectively. CONCLUSION A statistical comparative study conducted for the proposed methods with the high-performance liquid chromatography (HPLC) technique demonstrated no significant difference between these methods. The suggested approaches were simple, fast, accurate, and affordable. HIGHLIGHTS Three smart UV spectrophotometric methods based on chemometrics were presented to determine SIL and TM simultaneously.The efficient chemometrics methods, including CWT, PLS, and RBF-NN were used.The mentioned methods are rapid, simple, inexpensive, and accurate.The comparison was performed between the proposed models and HPLC technique.