LAUSR

We aimed to investigate the impact of maternal feed restriction at different stages of gestation on proteomic profile in the skeletal muscle of newborn goats. A total of 14 pregnant dams were randomly divided into one of the follow dietary treatments: Animals fed at 50% of maintenance requirement from 8-84 d of gestation and then fed at 100% maintenance requirement from day 85 of gestation to parturition (RM, n = 6), and animals fed at 100% of maintenance requirement from 8-84 d of gestation and then fed at 50% maintenance requirement from day 85 of gestation to parturition (MR, n = 8). Longissimus muscle was sampled from male newborn goats and submitted to sarcoplasmic protein extraction and liquid chromatography coupled to mass spectrometry (LC-MS) analysis. The raw data were processed with MaxQuant (1.6.3.3) software with parameters set to default values. Label-free quantification (LFQ) was added and only protein ratios calculated from at least two unique peptides were considered. Our data showed 3 differentially expressed proteins down-regulated in RM (q-value < 0.05). Additionally, we observed proteins present exclusively in each treatment (RM= 137 proteins; MR= 41 proteins). The overall enriched pathways in RM newborn goats are associated with glycolysis (PKM), NADPH synthesis (PGD), lipid oxidation (ECHS1, ACAT1) and citrate cycle (ACO1, OGDH). While the overall enriched pathways in MR newborn goats are associated with glycolysis/gluconeogenesis (GAPDH, ENO) and citrate cycle (PDHA, IDH3A). In addition, correlation analysis between shotgun proteomics and RNAseq data from the same samples showed that there were no relationships between proteins and transcripts observed. These results indicate that maternal feed restriction during different stages of gestation alters enzymes and protein domains abundance associated with nucleotide metabolism in the skeletal muscle of newborn goats. Moreover, the lack of correlation between protein-transcript suggests the importance of post-transcriptional regulation of skeletal muscle metabolism as a consequence of maternal feed restriction at different stages of gestation.