LAUSR

Effects of cyclic monensin feeding on in situ disappearance kinetics of low-quality forage (LQF; 4.9% CP) were evaluated. Ruminally cannulated steers (n = 12; Bos taurus; 260 kg BW) consuming LQF were randomly assigned to of 3 treatments in a completely randomized design: CON (0 mg∙animal-1∙day-1 monensin; Rumensin 90, Elanco Animal Health, Greenfield, IN), 2) MON (200 mg∙animal-1∙day-1 monensin), or 3) CYC (200 mg∙animal-1∙day-1 monensin for 4 d and 0 mg∙animal-1∙day-1 monensin for subsequent 4 d). Steers were fed dried distillers’ grains with solubles (1 kg∙animal-1∙day-1) for monensin inclusion. Four 28-d replicated cycles were used allowing 9 days for treatment adaptation, 4 days for sampling, and 4 days for withdrawal of monensin within the CYC treatment group. Low-quality forage samples were weighed into 0 × 20 cm polyester bags in replicates of four for each sampling hour. On d 0, all replicates were placed into the ventral area of the rumen and replicates were removed 0, 4, 8,6, 24, 48, and 72 hours post feeding. Upon removal, bags were rinsed in ice water, and frozen until final replicates were removed. Subsequently, bags were washed utilizing a commercial washing machine, dried at 55°C to a constant weight and dried residues were analyzed for DM. Ruminal in situ DM degradation data over time were fitted to the first-order exponential model with discrete lag using the NLIN procedure of SAS 9.4, then model parameters were compared with PROC GLIMMIX model to compare dietary treatments with period included as a random variable. There was no effect of treatment (P ≥ 0.22) on potentially degradable DM, rate of DM degradation, DM residue, DM lag, or wash loss of DM. Results are consistent with findings in the concurrent in vivo study conducted by Hook et al. (2022). Further evaluation of NDF degradability is required to determine a clear effect of cyclic monensin feeding on steers consuming an LQF.