LAUSR

The aim of this study was to evaluate the associated effects of ambient temperature and inflammation caused by repeated administration of Escherichia coli lipopolysaccharide (LPS) on insulin, energy and AA metabolism. Twent-eight pigs were assigned to one of two thermal conditions: thermoneutral (24°C) or high ambient temperature (30°C). The experimental period lasted 17 d, which was divided into a 7-d period without LPS (d -7 to d -1), and a subsequent 10-d LPS period (d 1 to 10) in which pigs were administered five repeated injections of LPS at 2-day intervals. Postprandial profiles of plasma insulin and nutrients were evaluated through serial blood samples taken on d -4 (P0), d 4 (P1), and d 8 (P2). Before the LPS-challenge (P0), postprandial concentrations of glucose, lactate, Gln, Ile, Leu, Phe, Tyr and Val were greater in pigs kept at 24 than at 30°C (P < 0.05). In contrast, Arg, Asp, Gly, His and Met postprandial concentrations at P0 were lower at 24 than at 30°C (P < 0.05). At both 24 and 30°C conditions, pigs had greater postprandial concentrations of insulin (P < 0.01) and lower concentrations of NEFA (P < 0.01) and α-amino nitrogen (P < 0.05) at P1 and P2 than at P0. Compared to P0, postprandial concentrations of glucose were greater (P < 0.05) at P1 in pigs kept at 24°C, and at P1 and P2 in pigs kept at 30°C. At both ambient temperatures, pigs had lower (P < 0.05) postprandial concentrations of Ala, Gly, His, Ile, Leu, Pro, Ser, Thr, Trp and Val at P1 and P2 than at P0. Arginine postprandial concentration at P1 was lower than at P0 in pigs kept at 24°C (P < 0.05), while no difference was observed in pigs at 30°C. Relative to P0, Gln and Tyr concentrations were lower at P1 and P2 in pigs kept at 24°C (P < 0.01); whereas lower Gln concentration was observed only at P2 (P < 0.01) and lower Tyr only at P1 (P < 0.01) in pigs kept at 30°C. Our study shows a hyperglycemic and hyperinsulinemic state in LPS-challenged pigs and a greater magnitude of this response in pigs kept at 30°C. Furthermore, LPS caused important changes in BCAA, His, Thr and Trp profiles suggesting the role these AA in supporting the inflammatory response. Finally, our results suggest that LPS-induced effects on postprandial profiles of specific AA (Arg, Gln, Phe and Tyr) may be modulated by ambient temperature.