Photo by nci from unsplash
Cell culture K562 cells were acquired from ATCC and maintained in Isocove’s Modified Dulbecco’s Media (IMDM) (Life Technologies) containing 10% fetal bovine serum. Adherent STAG2null lines were detached for subculture… Click to show full abstract
Cell culture K562 cells were acquired from ATCC and maintained in Isocove’s Modified Dulbecco’s Media (IMDM) (Life Technologies) containing 10% fetal bovine serum. Adherent STAG2null lines were detached for subculture using trypsin-EDTA (0.005% final concentration, Life Technologies). Differentiation to megakaryocytes was induced using 30 nM of phorbol 12myristate 13-acetate (Kuvardina et al., 2015) (PMA, Sigma). BET inhibitor JQ1 (catalogue #S7110; TOCRIS) was used at 500 nM (Bhagwat et al., 2016). Images of cells in culture were taken using x20 objective on an Olympus THP4.
Journal Title: Journal of Molecular Cell Biology
Year Published: 2020
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!