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SO025DCR2 ACCELERATES RENAL FIBROSIS AND TUBULAR CELL SENESCENCE BY PHOSPHORYLATING TYR 194 OF PRDX1 IN DIABETIC NEPHROPATHY

Cell senescence of renal tubular epithelial cells (RTECs), which is involved in renal tubulointerstitial fibrosis (TIF), is a key event in the progression of diabetic nephropathy (DN). However, the underlying… Click to show full abstract

Cell senescence of renal tubular epithelial cells (RTECs), which is involved in renal tubulointerstitial fibrosis (TIF), is a key event in the progression of diabetic nephropathy (DN). However, the underlying mechanism remains unclear. This study aims to investigate the role and mechanism of decoy receptor 2 (DcR2) in TIF and cell senescence of RTECs. Streptozotocin (STZ)-induced DN mice model and high glucose (HG)-induced cell senescent model were constructed. The expression of DcR2 were regulated by ultrasound-mediated gene transfer of DcR2-siRNA or DcR2-overexpression plasmid in vivo. The co-expression of DcR2 with senescent markers (p16, p21 and SA-β-gal) and fibrotic markers (FN, collagen I and α-SMA). The interaction of DcR2 and its ligand TRAIL and antagonistic receptor DR5 were detected by laser confocal and Co-IP. The DcR2-interaction proteins were screened in renal tissue of DN patients and HG-induced cells by Co-IP combining with LC-MS/MS. The interaction of DcR2 and PRDX1 was validated by Co-IP, pull down assay and immunofluorescence co-staining. Peroxidase activity of PRDX1 was assessed by the kits of reactive oxygen species (ROS) and specific 2-cys peroxidase activity. The level of PRDX1 phosphorylation was measured through WB and the phosphorylated site were studied by wild-type PRDX1 and mutant Tyr194 PRDX1. DcR2 was co-expressed with senescent markers and co-localization with fibrotic markers in DN patients and STZ-DN mice. Knockdown of DcR2 effectively decreased renal fibrosis and tubular cell senescence in STZ-induced DN mice. However, DcR2 overexpression showed the opposite effects. DcR2 didn’t interacted with TRAIL or DR5 in vivo and vitro. The following study indicated that PRDX1 is a novel DcR2-interacting protein using quantitative proteomics, which have peroxidase and antioxidant activity in cytoplasm. The interaction of DcR2-PRDX1 mediated RTEC senescence in vitro. Knockdown of DcR2 inhibited the level of ROS and promoted the peroxidase activity of PRDX1, resulting in oxidative stress and cell senescence, and DcR2 overexpression presented the contrary results. DcR2 regulated the level of PRDX1 phosphorylation by interacting Tyr194 of PRDX1, and the mutant Tyr194 of PRDX1 can’t be phosphorylated by DcR2. DcR2 mediates renal fibrosis and RTEC senescence by phosphorylating with PRDX1, suggesting DcR2 could serve as a potential therapeutic target for the amelioration of DN progression.

Keywords: dcr2; fibrosis; prdx1; cell senescence

Journal Title: Nephrology Dialysis Transplantation
Year Published: 2020

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