LAUSR

Abstract Eight ruminally cannulated beef steers were used to evaluate forage intake and physiological response during adaptation to a diet consisting of bermudagrass round bale silage (RBS) and dried distillers grains (DDG) and in a second experiment the effect of level of DDG supplementation on RBS forage intake, digestibility, and rumen parameters. The adaptation experiment was designed to simulate a 14-d step-up process. Initially steers received ad libitum RBS, on d 4 steers were offered 1.13 kg of DDG daily and every 4 d daily DDG offered was increased by 1.13 kg. Blood and rumen fluid samples were collected before feeding (0 h) and 6, 12, 24 (before feeding), and 36 h after every diet change. The digestibility experiment was conducted as an 8 × 4 Latin square with four periods. Treatments included RBS only and DDG supplement levels of 0.33%, 0.66%, and 1.0% of body weight (RBS + 0.33, +0.66, and 1.0, respectively). Collection periods consisted of 21 d to determine dry matter intake [DMI], digestibility, and collect rumen fluid samples. During adaptation, level of DDG did not (P = 0.42) affect RBS DMI. Mean ruminal pH was greater (P < 0.05) for RBS only compared with diets with DDG. When steers were offered 3.39 or 4.52 kg/d of DDG rumen NH3-N concentration was greater (P ≤ 0.05) compared with other levels of DDG or RBS alone. Mean nonesterified fatty acid and plasma urea N concentrations varied among levels of DDG offered. In the digestibility experiment forage DMI, total DMI, and total tract apparent digestibility exhibited a quadratic (P ≤ 0.05) response to level of DDG offered. Mean ruminal pH responded in a quadratic manner (P = 0.03) and was greater for RBS only compared with RBS plus DDG at any level. Mean ruminal NH3-N concentration tended (P = 0.07) to respond in a cubic manner as DDG amount offered increased. Physiological responses during adaptation to DDG over 14 d resembled responses previously observed after long-term DDG supplementation. Offering DDG up to 1% of body weight did depress forage DMI and ruminal pH but increased total DMI, diet digestibility, and ruminal NH3-N concentration.