LAUSR

Pinus elliottii, an important coniferous timber species, has recently become one of the most popular sources of resin in China. Resinosis is a common disease that may negatively affect pine tree growth and production. In this study, we used single molecule real-time sequencing and Illumina RNA sequencing to generate an accurate transcriptome for P. elliottii. The transcriptome included 90,026 transcripts, 5,160 long non-coding RNAs, and 7,710 transcription factors. We then analyzed RNA-sequencing, small RNA-sequencing, and degradome data to identify genes, miRNAs, and key miRNA-target pairs involved in response to resinosis in P. elliottii. We identified 1,305 genes and 1,151 miRNAs exhibiting significant differential expression in response to resinosis. According to the degradome sequencing analysis, 318 differentially expressed transcripts were targets of 14 differentially expressed miRNAs. Our study has provided resources for further functional characterization of genes and miRNAs involved in resinosis in P. elliottii that should aid future disease resistance breeding of this species.