LAUSR

Field pea (Pisum sativum L.) plants cultivar ‘Axe’ with symptoms of orthotospovirus infection (≈ 5% incidence) were collected under open field conditions in Brasilia-DF, Central Brazil. Ten leaf samples displaying apical chlorosis, necrosis, and deformation were evaluated via serology (ELISA) using antisera (produced at CNPH) specific to the nucleocapsid (N) protein of three Orthotospovirus species: Tomato chlorotic spot orthotospovirus (TCSV), Tomato spotted wilt orthotospovirus (TSWV), and Groundnut ringspot orthotospovirus (GRSV). ELISA tests indicated that all symptomatic pea plants as being positive for GRSV with some cross reaction with TCSV. RT-PCR assays were conducted to confirm the virus species infecting field pea. Total RNA from one pea infected sample (ER1 isolate) was extracted using TRIzol® and the purified nucleic acid preparation was used for RT-PCR assays. For cDNA synthesis, the enzyme M-MLV (Promega) was used with the J13 primer (5’-CCC-GGA-TCC-AGA-GCA-AT-3’), which has eight conserved...