London plane tree (Platanus × acerifolia) is an ornamental plant species of urban environments, and is widely planted in China and many other countries. In August 2014, symptoms of stem blight were… Click to show full abstract
London plane tree (Platanus × acerifolia) is an ornamental plant species of urban environments, and is widely planted in China and many other countries. In August 2014, symptoms of stem blight were observed on P. × acerifolia in Kunming, Yunnan province (southwestern China). Symptoms included stem dieback, and branch blight. The inner bark and the cambial region of the affected tissue were discoloured bluish gray; from 10 to 15% of trees were damaged. Samples from plants with symptoms were washed with running tap water, surface sterilised with 2% sodium hypochlorite and then 70% ethanol, rinsed in sterile distilled water, plated on potato dextrose agar (PDA) and incubated at 28°C. Fungal isolates developed copious white aerial mycelium that became dark grey after 4 to 6 days, and formed black pycnidia after 25 days. Conidia forming on PDA were one-celled, fusiform to ellipsoidal, externally smooth and thin walled, with dimensions of 16.0-23.0 x 5.2-7.8 (average 18.5 x 5.7 μm). Identity was confirmed by an...
               
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