LAUSR

The last two decades have seen exponential growth in the international movement of seeds for annual food crops, from a gross US import value of $349 million in 1999 to $1.05 billion in 2019. This has led to the proportionate growth of seed-borne pathogens dispersed with seed stocks. One such viral pathogen is cucumber green mottle mosaic virus (CGMMV), a tobamovirus that infects cucurbit crops such as melon, watermelon, cucumber, pumpkin, and squash. The first CGMMV introduction to California occurred in 2013, with subsequent annual outbreaks or detections since then. Here we describe the use of Next Generation Sequencing (NGS) to characterize the full genomes of 25 CGMMV isolates collected between 2013 and 2020 in California, either from CGMMV field detections or seed lots identified as CGMMV positive. We sequenced an additional 31 CGMMV isolates collected in Europe, Israel, and southeast Asia that were provided by industry collaborators. We also performed an in-silico nucleotide database search in GenBank for full genome CGMMV sequences to include in all in-silico analyses. Based on conserved regions within the coat protein gene, we then developed a quantitative reverse transcription polymerase chain reaction (RT-qPCR) assay for the sensitive and specific detection of CGMMV in seed and plant samples. Finally, based on our sequence and phylogenetic analysis, our data support that CGMMV has been introduced multiple times into California.