LAUSR

Bacterial spot disease (BSD) caused by Xanthomonas species is a global threat to tomato and pepper plants. A recent classification of these pathogens indicated the need for a diverse dataset of whole-genome resources. We report whole-genome resources of 89 Xanthomonas strains isolated from Canada (44), United States (30), Argentina (4), Brazil (3), Costa Rica (2), New Zealand (1), Australia (1), Mexico (1), Taiwan (1), Thailand (1) and unknown (1). Of these strains, 48 were previously identified to species-level based on non-genome-based approaches while 41 strains were classified only at the genus-level. The average coverage of the sequencing reads was 103x. The draft genome sizes ranged from 4.53-5.46 Mbp with a G+C content of 63.53 - 67.78% and comprised of 4,233-5,178 protein-coding sequences. Using average nucleotide identity (ANI) and genome-based DNA-DNA Hybridization (gDDH) values, the taxonomic classifications were validated for 38 out of the 48 strains previously assigned to species-level using other methods. Ten strains previously identified as X. campestris, X. axonopodis, X. vasicola and X. arboricola were incorrectly assigned, and new species-level delineations are proposed. Data from ANI, gDDH and pan-genome phylogeny of shared protein families were used to assign the 41 strains, previously identified only to genus-level, into 5 distinct species, X. euvesicatoria (pv. euvesicatoria or pv. perforans), X. hortorum pv. gardneri, X. vesicatoria, X. campestris and X. arboricola. These 89 whole-genome sequences of Xanthomonas strains, the majority (49.4%) of which are from Canada, could be useful resources in our understanding of global population structure and evolution of these pathogens.