LAUSR

First Report of a New, Unnamed Lesion Nematode Pratylenchus sp. Infecting Soybean in North Dakota | Plant Disease http://apsjournals.apsnet.org/doi/10.1094/PDIS-12-16-1749-PDN[7/13/2017 11:02:02 AM] 17.7 to 25.6), c’ (2.3, 2.2 to 2.4), spicule (17.5, 15.0 to 20.0), and gubernaculum (5.8, 4.5 to 7.5). DNA was extracted from single nematodes (n = 12) collected from soil and roots. D2-D3 of 28S rRNA (Subbotin et al. 2008) and ITS of rDNA (Yan and Smiley 2010) were amplified and sequenced. The ITS rDNA (KY200666, 981 bp) shared 95% sequence identity with P. alleni and low identity (≤87%) with other Pratylenchus spp. The D2-D3 (KY200665, 766 bp) had the highest identity of 96% with P. scribneri and 95% identity with P. hexincisus. No sequence of P. gibbicaudatus or P. flakkensis is available in GenBank. This species is very close to P. alleni but differs in having a slightly longer stylet, variations in tail shape, truncate to bluntly or broadly rounded tail with an annulated to occasionally smooth tail terminus, 4 to 6 lines in lateral field and 2 to 3 head annules. It is closely related to P. hexincisus, P. gibbicaudatus, and P. flakkensis but differs from them by one or more other characters: shape of head, number of head annules, tail shape, and V%. To our knowledge, this North Dakota Pratylenchus sp. population represents a new species of lesion nematode able to parasitize soybean.